John J.M. Bergeron, McGill University
Anatomy and Cell Biology
Professor
Montreal, Quebec
john.bergeron@mcgill.ca
Office:
(514) 398-1259
Expert In
Bio/Research
The lab uses proteomics to characterize the proteins of the mammalian cell by a strategy known as the CellMap. The cell is deconvoluted as a consequence of its constituent organelles which are isolated by exhaustive purification and presented to the tandem mass spectrometer. The tandem mass spect...
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Bio/Research
The lab uses proteomics to characterize the proteins of the mammalian cell by a strategy known as the CellMap. The cell is deconvoluted as a consequence of its constituent organelles which are isolated by exhaustive purification and presented to the tandem mass spectrometer. The tandem mass spectrometer is used much as a high resolution “protein” microscope to localize proteins quantitatively in the cell. In Gilchrist et al. (1), we were able to use a label free quantitative approach (2, 3) which considers the stochastic aspect of tandem mass spectrometry to localize quantitatively over 2000 different proteins.
Current work focuses on an extension of CellMap proteomics (4, 5) to organelles in different cell types which differ markedly in their morphological features and function in order to gain mechanistic insight into organelle identity, biogenesis and membrane trafficking mechanisms.
A benefit of proteomics is the uncovering of proteins of unknown function. Proteomics has enabled the localization of over 220 of these (324 have been found) and clustering methodologies have assigned each of these to predicted functional categories which are awaiting validation.
Click to Shrink <<
Current work focuses on an extension of CellMap proteomics (4, 5) to organelles in different cell types which differ markedly in their morphological features and function in order to gain mechanistic insight into organelle identity, biogenesis and membrane trafficking mechanisms.
A benefit of proteomics is the uncovering of proteins of unknown function. Proteomics has enabled the localization of over 220 of these (324 have been found) and clustering methodologies have assigned each of these to predicted functional categories which are awaiting validation.
Click to Shrink <<