Jonathan P. Staley, University of Chicago
Professor
Chicago, Illinois
jstaley@uchicago.edu
Office:
(773) 834-5886
Expert In
Bio/Research
Nuclear pre-mRNA splicing is ubiquitous in all eukaryotes and is required to excise introns before mRNA is translated by the ribosome. Further, splicing enables a critical layer in the regulation of gene expression as well as a flexibility through alternative splicing that affords complexity in h...
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Bio/Research
Nuclear pre-mRNA splicing is ubiquitous in all eukaryotes and is required to excise introns before mRNA is translated by the ribosome. Further, splicing enables a critical layer in the regulation of gene expression as well as a flexibility through alternative splicing that affords complexity in higher organisms, but splicing is also susceptible to mutations and underlies at least 15% of human diseases. Splicing is mediated by the spliceosome, a dynamic machine composed of both protein and noncoding RNA parts. Our lab investigates the role of these RNA components in substrate binding and catalysis, the mechanisms for proofreading splice site choice, the regulation of splicing, and the role for ATPase motors in dynamics and fidelity. Using budding yeast as a model system, we pursue these areas through a wide range of approaches, from single molecule microscopy and chemical biology to biochemistry, genetics, and genomics.
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