S. Murray Sherman, University of Chicago
Professor
Chicago, Illinois
msherman@bsd.uchicago.edu
Office:
(773) 834-2900
Bio/Research
The research in the laboratory is directed at issues of thalamic functional organization and thalamocortical relationships. We use a broad interdisciplinary approach, attempting to answer the same or closely related questions with several different techniques. More specifically, we use neuroanato...
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Bio/Research
The research in the laboratory is directed at issues of thalamic functional organization and thalamocortical relationships. We use a broad interdisciplinary approach, attempting to answer the same or closely related questions with several different techniques. More specifically, we use neuroanatomical techniques to explore various circuits; we use in vitro recordings from brain slices to study cell and synaptic properties; and we record from in vivo preparations to evaluate these circuits in whole animals with the ultimate plan to develop an awake, behaving preparation to determine the relationship between behavioral and cognitive parameters and thalamocortical functioning. Stimulation techniques in slices include electrical activation and laser photostimulation involving both uncaging of glutamate (or GABA) and optogenetics; recording involves mainly patching of single neurons and imaging via flavoprotein autofluorescence. For in vivo recording, we also plan to use electrical stimulation plus optogenetics (both ChR2 to activate and NpHR to inactivate based on Cre lines to control specific pathways) with recording via electrophysiology (single cell and current source density analysis) plus imaging via flavoprotein autofluorescence or intrinsic signals.
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